LAT抗体;LAT Antibody—艾普蒂

+ +

•  

   

     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Human lymph gland tissue lysate, Primary antibody: P12776(LAT Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P12776(LAT Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

LAT (Linker for Activation of T cells) is a transmembrane adaptor protein critical for T cell receptor (TCR)-mediated signaling. Discovered in the late 1990s, LAT serves as a scaffolding molecule that coordinates signal transduction by recruiting downstream effectors upon TCR engagement. It contains multiple tyrosine phosphorylation sites that, when phosphorylated, interact with signaling proteins like PLC-γ1. Grb2. and Gads, facilitating pathways such as calcium flux, MAPK activation, and cytoskeletal reorganization. LAT is essential for T cell development, activation, and effector functions, as evidenced by studies showing that LAT-deficient mice exhibit blocked T cell maturation and severe immunodeficiency.

Anti-LAT antibodies are vital tools in immunological research, enabling detection of LAT expression, phosphorylation status, and subcellular localization. They are widely used in Western blotting, immunofluorescence, and flow cytometry to study T cell signaling dynamics in health and disease. Dysregulated LAT expression or mutations have been linked to immune disorders, including certain primary immunodeficiencies and autoimmune conditions. In clinical contexts, LAT antibodies aid in diagnosing T cell malignancies, where aberrant signaling may correlate with LAT overexpression or phosphorylation anomalies. Additionally, these antibodies contribute to investigating LAT's role in non-T cells, such as mast cells and platelets, broadening its relevance in allergy and thrombosis research. Overall, LAT antibodies remain indispensable for unraveling T cell biology and related pathologies.