HSPB1抗体;HSPB1 Antibody—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Hepg2, A431, K562, Hela and 293T cell lysates, Primary antibody: P12748(HSPB1 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P12748(HSPB1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P12748(HSPB1 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

HSPB1 antibody targets the heat shock protein beta-1 (HSPB1), also known as HSP27. a member of the small heat shock protein (sHSP) family. This protein is ubiquitously expressed in human tissues and plays critical roles in cellular stress responses, acting as a molecular chaperone to facilitate proper folding of client proteins, prevent aggregation of denatured proteins, and promote cell survival under stress conditions. HSPB1 is regulated by phosphorylation, which modulates its oligomerization and chaperone activity. It is implicated in diverse cellular processes, including cytoskeletal stabilization, apoptosis inhibition, and oxidative stress defense.

Antibodies against HSPB1 are widely used in research to study its expression, localization, and function in both physiological and pathological contexts. In disease research, HSPB1 is associated with cancer progression (e.g., metastasis, chemoresistance), neurodegenerative disorders (e.g., Alzheimer’s, Charcot-Marie-Tooth disease), and inflammatory conditions. Its overexpression in certain cancers has prompted investigations into its potential as a diagnostic biomarker or therapeutic target.

Commercially available HSPB1 antibodies are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence. Researchers must consider post-translational modifications and tissue-specific isoforms when interpreting results. Proper controls, including knockout cell lines or siRNA knockdown, are recommended to confirm antibody specificity due to homology among sHSP family members.