MT-ND5抗体;MT-ND5 Antibody—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P12707(MT-ND5 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P12707(MT-ND5 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

The MT-ND5 antibody is a research tool designed to detect the MT-ND5 protein, a critical subunit of mitochondrial complex I (NADH:ubiquinone oxidoreductase) in the electron transport chain (ETC). Encoded by the mitochondrial genome (mtDNA), MT-ND5 plays a central role in oxidative phosphorylation (OXPHOS) by facilitating electron transfer and proton pumping across the inner mitochondrial membrane, essential for ATP production. Mutations in the MT-ND5 gene are linked to mitochondrial disorders, such as Leigh syndrome, mitochondrial encephalopathy, and optic neuropathy, as well as age-related degenerative conditions and cancer.

Researchers use MT-ND5 antibodies primarily in techniques like western blotting, immunohistochemistry, and immunofluorescence to study protein expression, subcellular localization, and mitochondrial dysfunction in disease models. These antibodies help identify pathogenic variants, assess mitochondrial integrity, and explore metabolic reprogramming in cancer or neurodegeneration.

Validation of MT-ND5 antibodies often involves knockout controls or tissue-specific expression profiles to confirm specificity, given the high homology among mitochondrial proteins. Challenges include cross-reactivity risks and the need for optimized protocols for mitochondrial membrane protein extraction. As mitochondrial research expands, MT-ND5 antibodies remain vital for unraveling the molecular basis of energy metabolism defects and developing targeted therapies.