KNL1抗体;KNL1 Antibody—艾普蒂 新品

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P12702(KNL1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P12702(KNL1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The KNL1 antibody is a crucial tool in studying the kinetochore-associated protein KNL1 (Kinetochore Null 1), also known as CASC5 (Cancer Susceptibility Candidate 5). KNL1 is a scaffold protein localized at the kinetochore, a multi-protein structure essential for proper chromosome segregation during mitosis and meiosis. It plays a pivotal role in the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring accurate chromosome attachment to spindle microtubules before anaphase. KNL1 recruits other SAC components, such as BUB1 and BUBR1. and facilitates the assembly of checkpoint signaling complexes. Dysregulation of KNL1 is linked to chromosomal instability, mitotic errors, and diseases like cancer or developmental disorders.

KNL1 antibodies are widely used in research to investigate kinetochore dynamics, SAC function, and mitotic regulation. They enable detection of KNL1 expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping to elucidate its interaction networks and post-translational modifications. Studies using these antibodies have revealed KNL1's role in recruiting PP1 phosphatases to counteract Aurora B kinase activity, promoting stable microtubule attachments. Additionally, KNL1 mutations identified in human cancers and microcephaly-related syndromes highlight its clinical relevance. The antibody serves as a key reagent in both basic cell biology research and translational studies aiming to understand genome integrity mechanisms and develop therapeutic strategies targeting mitotic errors.