ELK1抗体;ELK1 Antibody—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: TM4 cell lysate, Primary antibody: P12627(ELK1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 40 seconds    

  
  

The ELK1 antibody is a crucial tool in molecular biology for studying the ELK1 protein, a member of the ETS domain-containing transcription factor family. ELK1 plays a pivotal role in regulating gene expression by binding to specific DNA sequences, often in conjunction with serum response factor (SRF), to form ternary complexes at serum response elements (SREs). This interaction activates transcription of target genes involved in cell proliferation, differentiation, and apoptosis. ELK1 is phosphorylated and activated by mitogen-activated protein kinase (MAPK) signaling pathways, linking extracellular signals to nuclear transcriptional responses. Dysregulation of ELK1 has been implicated in cancer, neurological disorders, and cardiovascular diseases.

The ELK1 antibody is widely used in techniques like Western blotting, chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to detect ELK1 expression, localization, and DNA-binding activity. Researchers rely on its specificity to distinguish ELK1 from related ETS family members, such as SAP1 or NET. Antibodies targeting distinct epitopes (e.g., N-terminal transactivation domain or C-terminal DNA-binding domain) enable functional studies of ELK1’s modular structure. Validation via knockdown experiments or recombinant protein controls is essential to confirm antibody reliability. Commercial ELK1 antibodies are often raised in rabbits or mice, with clonal variants (monoclonal/polyclonal) offering flexibility for different applications. Its utility extends to both basic research and clinical investigations, particularly in exploring transcriptional networks and signaling pathways.