STARD4抗体;STARD4 Antibody—艾普蒂

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: HepG2 cell, Human liver tissue, Mouse liver tissue, Human fetal liver tissue, LO2 cell lysates, Primary antibody: P12247(STARD4 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P12247(STARD4 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P12247(STARD4 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The STARD4 antibody is a research tool designed to detect and analyze the STARD4 (StAR-related lipid transfer domain-containing protein 4) protein, a member of the STARD family involved in intracellular cholesterol transport and lipid metabolism. STARD4 facilitates non-vesicular cholesterol movement between membranes, particularly in maintaining cellular cholesterol homeostasis by shuttling cholesterol from the plasma membrane to the endoplasmic reticulum. Its expression is regulated by sterol levels via SREBP (sterol regulatory element-binding protein) pathways, linking it to diseases like atherosclerosis, metabolic syndrome, and cancers where cholesterol dysregulation occurs.

Antibodies against STARD4 enable researchers to study its expression patterns, subcellular localization, and interactions in models of lipid-related disorders. For example, they are used in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess STARD4 levels in tissues or cultured cells under varying sterol conditions. Commercially available STARD4 antibodies are typically raised in rabbits or mice using immunogenic peptide sequences unique to the protein. Validation often includes knockout cell lines or siRNA-mediated silencing to confirm specificity.

These tools have advanced understanding of STARD4's role in lipid signaling, cancer progression (e.g., hepatocellular carcinoma), and neurodegenerative diseases. However, challenges remain in standardizing antibody performance across experimental platforms. Ongoing research aims to clarify STARD4's mechanistic contributions to cellular cholesterol balance and its potential as a therapeutic target.