SMYD5抗体;SMYD5 Antibody—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: HT29 cells, 231 cells, hela cells, A549 cells, Primary antibody: P12221(SMYD5 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The SMYD5 antibody is a research tool designed to detect and study the SMYD5 protein, a member of the SMYD (SET and MYND domain-containing) family of lysine methyltransferases. SMYD5 contains a conserved SET domain responsible for methyltransferase activity and a MYND domain implicated in protein-protein interactions. Unlike other SMYD proteins (e.g., SMYD1-4), SMYD5 is less characterized but is proposed to play roles in epigenetic regulation, chromatin remodeling, and transcriptional repression. Studies suggest it may methylate histone H4 at lysine 20 (H4K20) or non-histone targets, influencing DNA repair, cell cycle progression, and immune responses. Dysregulation of SMYD5 has been linked to cancers, autoimmune disorders, and neurodegenerative diseases, though its precise mechanisms remain under investigation.

The SMYD5 antibody is typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. It is validated for applications such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specificity is confirmed via knockout controls or siRNA-mediated knockdown. Researchers use this antibody to explore SMYD5's expression patterns, subcellular localization (primarily nuclear), and interactions with chromatin-modifying complexes. Its role in diseases, particularly in oncogenesis and inflammation, makes it a focus for therapeutic targeting studies. Commercial SMYD5 antibodies vary in clonality, epitope recognition, and cross-reactivity across species (human, mouse, rat), necessitating careful selection based on experimental needs.