SLIT2抗体;SLIT2 Antibody—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse bladder tissue, Primary antibody: P12210(SLIT2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P12210(SLIT2 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P12210(SLIT2 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The SLIT2 antibody is a crucial tool in studying the SLIT2 protein, a member of the SLIT family of secreted glycoproteins. SLIT2 is best known for its role as a ligand for Roundabout (ROBO) receptors, mediating axon guidance, neuronal migration, and cell motility during embryonic development. Beyond neurodevelopment, SLIT2-ROBO signaling regulates diverse processes, including angiogenesis, immune cell migration, and cancer progression. In cancer, SLIT2 often acts as a tumor suppressor by inhibiting cell proliferation, invasion, and metastasis, though context-dependent pro-tumorigenic roles have also been reported.

SLIT2 antibodies are widely used in research to detect and quantify SLIT2 expression in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate SLIT2's spatial-temporal distribution, interaction partners, and dysregulation in diseases such as cancer, neurological disorders, and cardiovascular conditions. Polyclonal and monoclonal SLIT2 antibodies target specific epitopes, with validation often involving knockout controls or recombinant protein blocking to confirm specificity. Researchers also employ neutralizing SLIT2 antibodies to inhibit its function in vitro or in vivo, exploring therapeutic potential in pathological angiogenesis or metastasis. Challenges include ensuring antibody recognition of processed SLIT2 variants, as the full-length protein undergoes proteolytic cleavage into active N-terminal and C-terminal fragments. Commercial antibodies vary in species reactivity (human, mouse, rat), requiring careful selection based on experimental models. Recent studies highlight SLIT2's involvement in metabolic diseases and tissue repair, expanding the antibody's applications in translational research.