NDUFAF4抗体;NDUFAF4 Antibody—艾普蒂

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Jurkat cells, Hela cells, K562 cells, Primary antibody: P11972(NDUFAF4 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P11972(NDUFAF4 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The NDUFAF4 antibody is a crucial tool for studying the function and expression of the NDUFAF4 protein, a key player in mitochondrial complex I (NADH:ubiquinone oxidoreductase) assembly. NDUFAF4. also known as COX-associated protein 4 (COA4), is a nuclear-encoded mitochondrial matrix protein essential for the biogenesis of complex I, the largest enzyme in the electron transport chain. It interacts with other assembly factors, such as NDUFAF5 and ECSIT, to stabilize intermediate subunits during complex I maturation. Dysfunction of NDUFAF4 is linked to mitochondrial disorders, particularly Leigh syndrome, a severe neurodegenerative condition characterized by impaired energy metabolism.

Researchers use NDUFAF4 antibodies primarily in Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression levels, subcellular localization, and potential deficiencies in disease models. These antibodies aid in diagnosing mitochondrial pathologies and elucidating molecular mechanisms underlying complex I deficiencies. Commercially available NDUFAF4 antibodies are typically raised against specific epitopes, often validated in human or murine tissues, with cross-reactivity depending on species homology. Proper validation via knockout controls or siRNA knockdown is critical to ensure specificity, given the structural similarities among mitochondrial proteins. Studies employing this antibody contribute to advancing therapeutic strategies for mitochondrial diseases and understanding metabolic regulation in health and disease.