HSD11B2抗体;HSD11B2 Antibody—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human normal kidney tissue, Primary antibody: P11831(HSD11B2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P11831(HSD11B2 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P11831(HSD11B2 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The HSD11B2 antibody is a crucial tool for studying the enzyme 11β-hydroxysteroid dehydrogenase type 2 (HSD11B2), which plays a vital role in regulating cortisol metabolism. HSD11B2 converts active cortisol into inactive cortisone, preventing excessive activation of mineralocorticoid receptors in tissues like the kidneys, placenta, and colon. This enzymatic activity is essential for maintaining sodium-potassium balance and blood pressure homeostasis. Dysregulation of HSD11B2 is linked to hypertension, metabolic syndrome, and apparent mineralocorticoid excess (AME), a genetic disorder characterized by cortisol-induced mineralocorticoid hyperactivity.

Antibodies targeting HSD11B2 are widely used in research to detect protein expression, localization, and abundance in tissues or cell models. They enable techniques such as Western blotting, immunohistochemistry, and immunofluorescence, aiding investigations into HSD11B2's role in renal physiology, placental cortisol barrier function, and disease mechanisms. Most commercial HSD11B2 antibodies are raised in rabbits or mice using synthetic peptides or recombinant protein fragments as immunogens. Validation typically includes testing in knockout cell lines or siRNA-treated samples to confirm specificity. Researchers also utilize these antibodies to explore therapeutic interventions for HSD11B2-related disorders or to study epigenetic regulation of the HSD11B2 gene in metabolic diseases. Proper antibody validation remains critical due to potential cross-reactivity with homologous enzymes like HSD11B1.