pCDNA3.1(+)是来自pCDNA3的5.4kb载体，设计用于哺乳动物宿主中的高水平稳定和瞬时表达。大多数哺乳动物细胞可以进行高水平的稳定和非复制性瞬时表达。人类巨细胞病毒立即早期（CMV）启动子，用于在广泛哺乳动物细胞中的高水平表达。前向（+）和反向（-）方向上的多个克隆位点，以促进克隆用于选择稳定细胞系的新霉素抗性基因。在潜伏感染SV40或表达SV40大T抗原（例如COS-1，COS-7）的细胞系中的异常复制。
pcDNA3.1(+) and pcDNA3.1(-) are 5.4 kb vectors derived from pcDNA3 and designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements:
• Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells
• Multiple cloning sites in the forward (+) and reverse (-) orientations to facilitate cloning
• Neomycin resistance gene for selection of stable cell lines
• Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS-1, COS-7). The control plasmid, pcDNA3.1/CAT, is included for use as a positive control for transfection and expression in the cell line of choice.
This pcDNA3.1(+)vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin® selectable marker and a forward-orientation multiple cloning site.
质粒序列：
1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
901 cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgagtct
961 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
1021 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
1081 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
1141 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
1201 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
1261 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
1321 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
1381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
1441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
1501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
1561 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
1621 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
1681 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
1741 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
1801 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
1861 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
1921 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
1981 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
2041 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
2101 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
2161 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
2221 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
2281 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
2341 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
2401 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
2461 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
2521 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
2581 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
2641 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
2701 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
2761 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
2821 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
2881 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
2941 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
3001 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
3061 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
3121 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
3181 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
3241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
3301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
3361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
3421 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
3481 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
3541 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
3601 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
3661 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
3721 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
3781 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
3841 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
3901 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
3961 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
4021 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
4081 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
4141 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
4201 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4261 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4321 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
4381 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
4441 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
4501 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
4561 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
4621 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
4681 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
4741 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
4801 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
4861 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
4921 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
4981 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
5041 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5101 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5161 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5221 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
5281 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
5341 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
5401 atcgggagat ctcccgatcc cctatggt
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