pCDNA3.0载体是专门设计用来在哺乳细胞中进行高水平的稳定表达和瞬时表达。其中人巨细胞病毒早期启动子（CMV）在广泛的哺乳动物细胞中可以实现高表达。另外为筛选稳定细胞系提供了新霉素抗性基因，可用G418筛选。
pcDNA3.0 is 5.4 kb vectors designed for high-level stable and transient expression in mammalian hosts. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells. Neomycin resistance gene for selection of stable cell lines.
pcDNA3.0 vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin selectable marker and a forward-orientation multiple cloning site. High-level stable and non-replicative transient expression can be carried out in most mammalian cells. The vectors contain the following elements: Human cytomegalovirus immediate-early (CMV) promoter for high-level expression in a wide range of mammalian cells Multiple cloning sites orientations to facilitate cloning .Neomycin resistance gene for selection of stable cell lines Episomal replication in cells lines that are latently infected with SV40 or that express the SV40 large T antigen.
质粒序列：
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat atccatcaca
961 ctggcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
1021 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
1081 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
1141 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
1201 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
1261 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
1321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
1381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
1441 gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac
1501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
1561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
1621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
1681 tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
1741 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca
1801 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
1861 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
1921 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
1981 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
2041 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
2101 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
2161 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
2221 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
2281 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
2341 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
2401 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
2461 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
2521 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
2581 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
2641 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
2701 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
2761 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
2821 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
2881 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
2941 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
3001 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
3061 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
3121 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
3181 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
3241 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
3301 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
3361 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
3421 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
3481 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
3541 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
3601 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
3661 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
3721 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
3781 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
3841 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
3901 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
3961 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
4021 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
4081 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
4141 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
4201 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
4261 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
4321 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
4381 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
4441 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
4501 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
4561 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
4621 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
4681 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
4741 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
4801 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
4861 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
4921 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
4981 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
5041 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
5101 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
5161 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
5221 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
5281 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
5341 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
5401 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
