FireflyLuciferase抗体—艾普蒂 新品

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     Western blot analysis of Firefly Luciferase Antibody expression in (1) Firefly Luciferase transfected 293T cell lysate; (2) HeLa cell lysate; (3) NIH/3T3 cell lysate; (4) C6 cell lysate.    

  
  

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     Immunofluorescent analysis of 293 cells transfected with Firefly Luciferase, using Firefly Luciferase Antibody .    

  
  

Firefly luciferase (FLuc) antibody is a crucial tool in molecular and cellular biology, primarily used to detect and quantify the presence of firefly luciferase, an enzyme derived from the North American firefly (*Photinus pyralis*). FLuc catalyzes the oxidation of D-luciferin in the presence of ATP and oxygen, producing light (bioluminescence) that serves as a sensitive reporter in various assays. FLuc antibodies are widely employed in techniques like Western blotting, ELISA, immunoprecipitation, and immunofluorescence to monitor FLuc-tagged proteins, gene expression patterns, or transgenic models.

These antibodies are typically raised against purified FLuc protein or specific epitopes, available as monoclonal or polyclonal formats. Monoclonal antibodies offer high specificity, ideal for distinguishing FLuc from other luciferases (e.g., Renilla luciferase) in dual-reporter systems, while polyclonal antibodies may provide higher sensitivity due to multiple epitope recognition. FLuc antibodies are critical in drug discovery, promoter activity studies, and protein trafficking research, where precise quantification of reporter activity is essential. Validation often involves testing against FLuc-expressing cell lysates or recombinant proteins to confirm specificity and absence of cross-reactivity. Their reliability ensures accurate normalization and interpretation in experiments leveraging FLuc-based bioluminescence imaging or high-throughput screening.