CaMKII抗体—艾普蒂

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.    

  
  

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     Western blot analysis of CaMKII expression in (1)Mouse brain tissue lysate ;(2)Rat brain tissue lysate.    

  
  

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     Immunofluorescent analysis of PC-12 cells, using CaMKII Antibody .    

  
  

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     Immunohistochemical analysis of paraffin-embedded mouse colon, using CaMKII Antibody.    

  
  

CaMKII (Calcium/Calmodulin-dependent Protein Kinase II) antibodies are essential tools in neuroscience and cardiovascular research, targeting a multifunctional serine/threonine kinase critical for synaptic plasticity, memory formation, and cardiac function. CaMKII is activated by calcium influx, which triggers its binding to calmodulin, leading to autophosphorylation at Thr286 (α-isoform) or Thr287 (β, γ, δ-isoforms). This modification sustains kinase activity even after calcium levels drop, enabling long-term signaling. Structurally, CaMKII forms a dodecameric holoenzyme composed of homologous subunits (α, β, γ, δ), with tissue-specific expression—α and β dominate in the brain, while δ is prominent in the heart.

Antibodies against CaMKII are widely used to study its expression, localization, and activation states. Phospho-specific antibodies targeting autophosphorylation sites (e.g., Thr286/287) help assess kinase activation in conditions like long-term potentiation (LTP), heart failure, or arrhythmias. These antibodies are applied in techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Challenges include cross-reactivity due to high homology among isoforms, necessitating rigorous validation via knockout controls or isoform-specific assays.

Research areas leveraging CaMKII antibodies range from neurodegenerative diseases (e.g., Alzheimer’s) to cardiac hypertrophy, underscoring its role in cellular signaling. Recent studies also explore its non-catalytic functions in structural scaffolding. Monoclonal antibodies offer consistency, while polyclonal ones may detect broader epitopes. Proper experimental design requires matching antibody specificity to the target isoform and post-translational modification, ensuring accurate interpretation of CaMKII's complex roles in health and disease.