Actin(Alpha-Actin)抗体—艾普蒂 新品

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     Western blot analysis of Actin (Alpha-Actin) expression in (1) A431 cell lysate; (2) MCF-7 cell lysate.    

  
  

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     Immunofluorescent analysis of Hela cells, using Actin (Alpha-Actin) Antibody .    

  
  

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     Immunohistochemical analysis of paraffin-embedded mouse muscle, using Actin (Alpha-Actin) Antibody.    

  
  

Actin, a highly conserved cytoskeletal protein, plays a fundamental role in maintaining cell structure, motility, and division across eukaryotic organisms. The α-actin isoform (Alpha-Actin) is predominantly expressed in muscle cells, including skeletal, cardiac, and smooth muscles, where it forms the core of thin filaments essential for muscle contraction. Its sequence differs slightly from cytoplasmic β- and γ-actin isoforms, enabling isoform-specific functional specialization.

Alpha-Actin antibodies are widely used in research to identify and study muscle-specific actin expression patterns. These antibodies are critical tools in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to validate skeletal/cardiac muscle differentiation, assess muscle pathology (e.g., myopathies), or confirm tissue specificity in experimental models. They often serve as loading controls in muscle-related studies due to their consistent expression in muscle tissues.

Commercially available Alpha-Actin antibodies typically recognize epitopes within the N-terminal region of the protein and exhibit cross-reactivity with human, mouse, and rat samples. However, researchers must verify antibody specificity, as actin isoforms share >90% sequence homology. Proper validation using knockout controls or isoform-specific assays is recommended to avoid cross-reactivity artifacts. These antibodies have advanced studies in muscle development, regeneration, and disease mechanisms, including muscular dystrophy and cardiovascular disorders.