FTCD抗体—艾普蒂

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     96-well microtitre plates were coated with 1 µg/mL of FTCD protein at 4 °C overnight. Plates were blocked with 2 % BSA in PBS-T (0.1 % Tween) at room temperature for 1 hour. Serial diluted of P16608 was used as the primary antibody and added into the plates for 1 hour incubation. Plates were washed with PBS-T for three times and then incubated with HRP conjugated Human IgG1 for 1 hour. After washed with PBS-T for three times, the plates were incubated with ABTS for 15 minute. The absorbance (optical density) was read with a plate reader.    

  
  

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     Western blot analysis using FTCD mAb against  Hep G2 lysate    

  
  

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     IHC-P analysis of liver tissue by FTCD antibody (P16608) IHC-P was performed using sections of the formalin-fixed paraffin-embedded liver tissue;Result: Hepatocytes are positively stained at the cytoplasm.    

  
  

**Background of FTCD Antibodies**

Formimidoyltransferase cyclodeaminase (FTCD) is a bifunctional enzyme critical in histidine metabolism and the folate cycle, facilitating the conversion of formiminoglutamate to glutamate and formate. FTCD is predominantly expressed in the liver and kidneys, where it localizes to mitochondria and the cytoplasm. Its role in metabolic pathways links it to various disorders, particularly liver diseases.

FTCD has gained attention as an autoantigen in autoimmune hepatitis (AIH), specifically type 2 AIH, where anti-FTCD antibodies are detected alongside anti-liver-kidney microsomal type 1 (anti-LKM1) antibodies. These autoantibodies are diagnostic markers, aiding in distinguishing AIH from other liver conditions. Additionally, FTCD dysfunction is implicated in hepatocellular carcinoma (HCC), with studies suggesting its downregulation may promote tumor progression by altering folate metabolism and mitochondrial function.

Antibodies targeting FTCD are essential tools in research and diagnostics. They enable the detection of FTCD expression in tissues (e.g., via immunohistochemistry) and quantification in serum (e.g., ELISA). In research, FTCD antibodies help elucidate its metabolic roles and interactions in disease pathogenesis. However, the exact mechanisms linking FTCD autoimmunity to AIH remain unclear, warranting further investigation. Overall, FTCD antibodies serve as valuable biomarkers and reagents for studying liver-related metabolic and autoimmune disorders.

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