SAP18抗体—艾普蒂

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     IHC-P analysis of testis tissue by SAP18 antibody (P16371) IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue;Result: Cells in seminiferous ducts are positively stained at the nuclei.    

  
  

The SAP18 (Sin3A-associated protein 18) antibody is a research tool used to study the SAP18 protein, a component of the SIN3 transcriptional regulatory complex. SAP18. with a molecular weight of approximately 18 kDa, plays a role in gene silencing by recruiting histone deacetylases (HDACs) to chromatin, facilitating chromatin condensation and transcriptional repression. It interacts with SIN3. HDACs, and other co-repressors, linking epigenetic modifiers to sequence-specific DNA-binding proteins.

SAP18 is implicated in diverse cellular processes, including apoptosis, RNA splicing, and stress responses. Studies highlight its involvement in developmental regulation, cancer (via tumor suppressor pathways), and neurological disorders. Dysregulation of SAP18-associated complexes may contribute to aberrant gene expression in diseases.

The SAP18 antibody is widely used in techniques like Western blotting, immunoprecipitation, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interactions. Researchers utilize it to explore SAP18's role in epigenetic regulation, particularly in contexts like DNA damage response or differentiation. Commercial SAP18 antibodies are typically validated for specificity, often targeting conserved regions across species. However, batch variability and cross-reactivity with homologous proteins require careful experimental controls. Its applications span molecular biology, cancer research, and neurobiology, offering insights into chromatin remodeling mechanisms.