PRKAR1A抗体—艾普蒂

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     IHC-P analysis of testis tissue by PRKAR1A antibody (P16033) IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue;Result: Cells in seminiferous ducts are positively stained at the cytoplasm and cell membrane.    

  
  

The PRKAR1A antibody is a crucial tool for studying the regulatory subunit 1α of protein kinase A (PKA), a central component of the cAMP-dependent signaling pathway. PRKAR1A (Protein Kinase cAMP-Dependent Regulatory Type I Alpha) binds to catalytic subunits of PKA, maintaining their inactive state until cAMP activation. This subunit plays a vital role in cellular processes, including metabolism, gene expression, and apoptosis.

Mutations in the PRKAR1A gene are linked to Carney complex, a rare genetic disorder characterized by endocrine tumors, skin pigmentation, and cardiac myxomas. Researchers use PRKAR1A antibodies to investigate protein expression, localization, and dysfunction in disease models, particularly in tumorigenesis and hormone-related pathologies.

These antibodies are widely applied in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect PRKAR1A in tissues or cell lines. Specificity varies; some target the N-terminal region, while others recognize epitopes near phosphorylation sites. Validation often includes knockout controls or siRNA-mediated silencing to confirm target binding.

Commercial PRKAR1A antibodies are available from multiple suppliers, with applications spanning cancer research, endocrine studies, and molecular pathways involving cAMP signaling. Proper experimental optimization (e.g., fixation methods, antigen retrieval) is critical for accuracy, especially in archival clinical samples. Ongoing research continues to explore PRKAR1A's role in cellular regulation and its potential as a therapeutic target.