ZNF174抗体—艾普蒂

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     IHC-P analysis of testis tissue by ZNF174 antibody (P16006) IHC-P was performed using sections of the formalin-fixed paraffin-embedded testis tissue;Result: Cells in seminiferous ducts are positively stained at the nuclei.    

  
  

The zinc finger protein 174 (ZNF174), also known as ZNF306 or ZKSCAN4. is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family. These proteins are characterized by tandem C2H2-type zinc finger motifs that bind DNA, enabling sequence-specific transcriptional regulation. ZNF174 is implicated in diverse cellular processes, including chromatin remodeling, cell cycle control, and tumor suppression. Studies suggest its potential role in repressing gene expression by recruiting chromatin-modifying complexes through its KRAB domain.

ZNF174 antibodies are immunological tools designed to detect and study this protein in experimental settings. These antibodies are typically developed in host species like rabbits or mice using immunogenic epitopes derived from ZNF174's unique regions, such as its N-terminal KRAB domain or C-terminal zinc finger clusters. They are widely used in techniques including Western blotting, immunofluorescence, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP) to investigate ZNF174's expression patterns, subcellular localization (primarily nuclear), and molecular interactions.

Commercially available ZNF174 antibodies are often validated for specificity using knockout cell lines or siRNA-mediated knockdown. Research utilizing these antibodies has linked ZNF174 to cancer biology, particularly in hepatocellular carcinoma and colorectal cancer, where it may act as a tumor suppressor by modulating pathways like p53 signaling. However, the functional landscape of ZNF174 remains incompletely mapped, driving continued demand for reliable antibodies to explore its physiological and pathological roles.