FGF3抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Rat liver tissue lysate, Primary antibody: P10720(FGF3 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P10720(FGF3 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P10720(FGF3 Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

Fibroblast Growth Factor 3 (FGF3) is a member of the FGF family, which comprises 22 structurally related proteins regulating cell proliferation, differentiation, migration, and survival. FGF3. initially identified as a proto-oncogene (INT2) in mouse mammary tumors, binds to FGF receptors (FGFRs) with heparan sulfate proteoglycans as cofactors. It plays critical roles in embryonic development, particularly in inner ear morphogenesis, tooth bud formation, and limb patterning. Dysregulation of FGF3 is linked to developmental disorders and cancers, such as breast, colorectal, and hepatocellular carcinomas, where it may drive tumor growth, angiogenesis, or metastasis.

FGF3 antibodies are essential tools for detecting and quantifying FGF3 expression in research and diagnostics. They are used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and ELISA to study FGF3's spatial-temporal expression, interactions, and signaling pathways. Polyclonal antibodies target multiple epitopes, offering high sensitivity, while monoclonal antibodies provide specificity for precise applications. These antibodies aid in exploring FGF3's role in disease mechanisms, evaluating its potential as a therapeutic target, or assessing drug efficacy in preclinical models. However, cross-reactivity with other FGF family members remains a challenge, necessitating rigorous validation using knockout controls or epitope mapping. Commercial FGF3 antibodies are typically raised in rabbits or mice, with recombinant or peptide antigens used for immunization. Proper sample preparation and antigen retrieval are critical for optimal performance, especially in FFPE tissues. Ongoing research continues to refine antibody specificity and expand their applications in both basic and translational studies.