ARFIP2抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10621(ARFIP2 Antibody) at dilution 1/70. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P10621(ARFIP2 Antibody) at dilution 1/70. (Original magnification: ×200)    

  
  

ARFIP2 (ADP-ribosylation factor interacting protein 2) is a protein involved in regulating intracellular trafficking and cytoskeletal dynamics by interacting with Arf GTPases, which are critical for vesicle formation and membrane trafficking. It contains a PH (pleckstrin homology) domain that mediates lipid binding and facilitates its recruitment to cellular membranes. ARFIP2 is implicated in processes like endocytosis, Golgi organization, and cell migration, with potential roles in cancer metastasis and neurological disorders.

Antibodies targeting ARFIP2 are essential tools for studying its expression, localization, and function. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. Validation methods include Western blotting (detecting ~40 kDa band), immunofluorescence (showing cytoplasmic or membrane-associated staining), and immunohistochemistry. Specificity is confirmed via knockout/knockdown controls or competitive inhibition assays.

ARFIP2 antibodies are utilized in research exploring its interaction with Arf proteins (e.g., ARF1. ARF6) and downstream signaling pathways. Dysregulation of ARFIP2 has been linked to diseases such as glioblastoma and Alzheimer’s, making these antibodies valuable for mechanistic and diagnostic studies. Commercial products often provide datasheets detailing application-specific protocols, recommended dilutions, and storage conditions (e.g., -20°C in glycerol-containing buffers). Proper controls are critical due to potential cross-reactivity with homologous proteins like ARFIP1.