SPTLC2抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A549 and HT29 cell lysates, Primary antibody: P10570(SPTLC2 Antibody) at dilution 1/900, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P10570(SPTLC2 Antibody) at dilution 1/120. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P10570(SPTLC2 Antibody) at dilution 1/120. (Original magnification: ×200)    

  
  

The SPTLC2 antibody is a research tool designed to detect serine palmitoyltransferase long-chain base subunit 2 (SPTLC2), a critical enzyme in sphingolipid biosynthesis. SPTLC2. along with SPTLC1 or SPTLC3. forms the heterodimeric serine palmitoyltransferase (SPT) complex, which catalyzes the first and rate-limiting step in sphingolipid production: the condensation of serine and palmitoyl-CoA to generate 3-ketodihydrosphingosine. Dysregulation of SPTLC2 is linked to metabolic disorders, neurodegenerative diseases, and hereditary sensory and autonomic neuropathy type 1 (HSAN1), often caused by mutations in the SPTLC1/SPTLC2 genes.

SPTLC2 antibodies are widely used in Western blotting, immunohistochemistry, and immunofluorescence to study protein expression, localization, and regulation in tissues or cell lines. These antibodies help elucidate sphingolipid metabolism’s role in cellular processes like apoptosis, membrane integrity, and signal transduction. Commercially available SPTLC2 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the human SPTLC2 protein. Validation includes testing for specificity via knockout controls or siRNA-mediated knockdown. Researchers also employ these antibodies to explore therapeutic strategies targeting sphingolipid pathways in cancer, metabolic syndromes, and neurological disorders. Proper controls and standardized protocols are essential to ensure reproducibility, given the potential cross-reactivity with homologous subunits like SPTLC3.