FBXO34抗体—艾普蒂

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-2: SKOV3 and 293T cell lysates, Primary antibody: P10531(FBXO34 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    

  
  

The FBXO34 antibody is a research tool designed to detect and study the F-box protein 34 (FBXO34), a member of the F-box protein family. FBXO34 is part of the SCF (SKP1-CUL1-F-box) ubiquitin ligase complex, which plays a critical role in the ubiquitin-proteasome system by targeting specific substrates for degradation. Structurally, FBXO34 contains an F-box domain that facilitates interactions with SKP1. enabling substrate recognition and recruitment. This protein is implicated in diverse cellular processes, including cell cycle regulation, DNA damage response, and signal transduction, though its exact physiological roles remain under investigation.

Antibodies against FBXO34 are commonly used in techniques such as Western blotting, immunohistochemistry (IHC), and immunoprecipitation (IP) to analyze protein expression, localization, and interactions. They are essential for exploring FBXO34's involvement in diseases, particularly cancer, where dysregulation of ubiquitination pathways is often observed. For example, studies suggest FBXO34 may influence tumor progression by modulating oncogenic or tumor-suppressive substrates.

Commercial FBXO34 antibodies are typically raised in hosts like rabbits or mice, with validation steps including knockout cell line controls to confirm specificity. Researchers rely on these antibodies to uncover FBXO34's molecular mechanisms, substrate profiles, and therapeutic potential. Despite progress, challenges persist in fully characterizing its biological contexts, underscoring the continued need for high-quality reagents like FBXO34 antibodies in both basic and translational research.