RILPL1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse heart tissue, Mouse brain tissue lysates, Primary antibody: P10505(RILPL1 Antibody) at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10505(RILPL1 Antibody) at dilution 1/75. (Original magnification: ×200)    

  
  

The RILPL1 (Rab-interacting lysosomal protein-like 1) antibody is a tool used to study the function and localization of the RILPL1 protein, a member of the RILP (Rab-interacting lysosomal protein) family. RILPL1 is involved in intracellular trafficking and cytoskeletal organization, primarily through its interaction with Rab GTPases, particularly Rab8 and Rab36. These interactions regulate vesicle transport, ciliogenesis, and cell division. RILPL1 is structurally characterized by an N-terminal coiled-coil domain and a conserved C-terminal region shared with RILP, enabling Rab binding and effector functions.

Research using RILPL1 antibodies has highlighted its role in ciliary biology and mitosis. Dysregulation of RILPL1 is implicated in ciliopathies, mitotic defects, and cancers, making it a focus in studies of cellular dynamics and disease mechanisms. The antibody is commonly applied in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect protein expression, subcellular distribution (e.g., centrosomes, cilia base), and interaction partners.

Commercial RILPL1 antibodies are typically developed in rabbits or mice, with validation in specific cell lines or tissues. Challenges include ensuring specificity due to homology with RILP and cross-reactivity risks. Recent studies utilizing these antibodies have advanced understanding of RILPL1's regulatory mechanisms in Rab-mediated trafficking and its potential as a therapeutic target in cilia-related disorders.