DHX8抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P10479(DHX8 Antibody) at dilution 1/100. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P10479(DHX8 Antibody) at dilution 1/100. (Original magnification: ×200)    

  
  

The DHX8 antibody is a research tool designed to target the DEAH-box helicase 8 (DHX8) protein, a member of the DEAH-box RNA helicase family involved in pre-mRNA splicing and RNA metabolism. DHX8 plays a critical role in the later stages of spliceosome assembly, facilitating the release of mature mRNA from the spliceosome and ensuring proper RNA processing. Its function is essential for maintaining genomic stability and regulating gene expression.

Antibodies against DHX8 are widely used in molecular biology to study its expression, localization, and interaction partners in cellular pathways. They enable techniques like Western blotting, immunofluorescence, and immunoprecipitation, helping researchers investigate DHX8's role in diseases linked to splicing defects, such as cancer, neurodegenerative disorders, or viral infections. Some studies also explore DHX8's involvement in cellular stress responses or its potential as a therapeutic target.

These antibodies are typically raised in rabbits or mice using recombinant DHX8 protein fragments or synthetic peptides. Validation often includes testing in knockout cell lines to confirm specificity. Commercial DHX8 antibodies vary in clonality (monoclonal/polyclonal) and conjugates (e.g., HRP, fluorescent tags). Researchers must optimize experimental conditions due to potential cross-reactivity with related helicases. Recent applications include CRISPR-based studies and high-throughput screening for splicing-related drug discovery.