IQSEC1抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10466(IQSEC1 Antibody) at dilution 1/125. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P10466(IQSEC1 Antibody) at dilution 1/125. (Original magnification: ×200)    

  
  

The IQSEC1 antibody is a crucial tool in neuroscience and cell biology research, targeting the IQ motif and SEC7 domain-containing protein 1 (IQSEC1), also known as BRAG2. This protein belongs to the ADP-ribosylation factor guanine nucleotide exchange factor (ArfGEF) family, which regulates intracellular trafficking and signaling by activating Arf GTPases. IQSEC1 specifically interacts with postsynaptic scaffolding proteins, influencing synaptic plasticity, dendritic spine morphology, and neurotransmitter receptor trafficking in neurons.

Researchers use IQSEC1 antibodies primarily in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study its expression, localization, and functional roles. These antibodies are typically raised in rabbits or mice, with both monoclonal and polyclonal variants available. Validation often includes knockout controls or siRNA knockdown to confirm specificity, given potential cross-reactivity with homologous family members (e.g., IQSEC2/IQSEC3).

Recent studies highlight IQSEC1's involvement in neurological disorders, including intellectual disability and autism spectrum disorders, as well as its emerging role in cancer cell migration. Antibodies targeting specific domains (e.g., SEC7 or IQ motifs) help dissect its molecular interactions, particularly with calcium/calmodulin signaling components or Arf GTPases. Optimal antibody performance may depend on sample preparation, as IQSEC1’s activity and phosphorylation status vary across tissues and developmental stages. Proper validation remains essential for interpreting its complex roles in health and disease.