PAIP1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 293T and HepG2 cell lysates, Primary antibody: P10437(PAIP1 Antibody) at dilution 1/1150, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P10437(PAIP1 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P10437(PAIP1 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

The PAIP1 (Poly(A)-Binding Protein-Interacting Protein 1) antibody is a research tool designed to detect and study the PAIP1 protein, a key regulator of mRNA translation and stability. PAIP1 interacts with poly(A)-binding protein (PABP), enhancing the circularization of mRNA and promoting ribosome recruitment during translation initiation. It contains three conserved domains: PAM2 (mediates PABP binding), PABC (interacts with proteins like PAIP2), and MA3 (binds eukaryotic translation initiation factor 4G, eIF4G). Dysregulation of PAIP1 is implicated in cancer, neurodevelopmental disorders, and viral infections due to its role in modulating protein synthesis.

PAIP1 antibodies are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate its expression, localization, and interactions in cellular models. These antibodies help elucidate PAIP1's dual roles in stimulating or repressing translation, depending on cellular context and binding partners. Commercial PAIP1 antibodies are typically raised in rabbits or mice, with specificity validated via knockout controls. Research applications include studying mRNA metabolism, stress granule dynamics, and diseases linked to translational dysregulation. Proper antibody selection depends on experimental needs, including epitope regions, host species, and cross-reactivity data.