ARPC1B抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Hela cell lysate, Primary antibody: P10416(ARPC1B Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P10416(ARPC1B Antibody) at dilution 1/65. (Original magnification: ×200)    

  
  

The ARPC1B antibody targets the Actin-Related Protein 2/3 Complex Subunit 1B (ARPC1B), a key component of the Arp2/3 complex, which regulates actin polymerization and cytoskeletal reorganization in eukaryotic cells. The Arp2/3 complex is essential for forming branched actin networks, a process critical for cell motility, membrane protrusion, and intracellular trafficking. ARPC1B, one of two isoforms (alongside ARPC1A), is predominantly expressed in hematopoietic cells and plays a specialized role in immune cell functions, including leukocyte migration and immunological synapse formation.

Antibodies against ARPC1B are widely used to study its expression, localization, and interactions in cellular processes. They are applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate ARPC1B’s involvement in diseases linked to cytoskeletal dysregulation. For instance, ARPC1B deficiency is associated with platelet abnormalities, combined immunodeficiency, and autoimmune disorders, making its antibody a vital tool for diagnosing and researching these conditions.

Additionally, ARPC1B-specific antibodies help distinguish between ARPC1A and ARPC1B isoforms, which share ~65% amino acid identity but differ in tissue distribution and functional roles. Researchers use these antibodies to explore isoform-specific mechanisms in cancer metastasis, inflammation, and immune cell signaling. Validating antibody specificity is crucial, as cross-reactivity with ARPC1A may occur. Overall, ARPC1B antibodies are indispensable for dissecting cytoskeletal dynamics in both basic cell biology and clinical research contexts.