GNAI1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A172 and Hela cell lysates, Primary antibody: P10401(GNAI1 Antibody) at dilution 1/2000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P10401(GNAI1 Antibody) at dilution 1/130. (Original magnification: ×200)    

  
  

The GNAI1 antibody is a crucial tool in studying the G protein subunit alpha i1 (GNAI1), a member of the inhibitory Gαi/o subfamily of heterotrimeric G proteins. GNAI1 plays a pivotal role in signal transduction by coupling G protein-coupled receptors (GPCRs) to downstream effectors, primarily through inhibiting adenylate cyclase activity and regulating ion channels, cell proliferation, and differentiation. Dysregulation of GNAI1 has been implicated in various pathologies, including cancers, neurological disorders, and cardiovascular diseases.

Antibodies targeting GNAI1 enable researchers to detect, quantify, and localize the protein in cells and tissues using techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are typically validated for specificity and affinity, often through knockout controls or siRNA-based silencing. Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies provide high consistency.

GNAI1 antibodies are widely used in basic research to explore GPCR signaling mechanisms, disease pathways, and therapeutic targets. In cancer studies, they help assess GNAI1's role in tumor progression or suppression, as its expression varies across malignancies. In neuroscience, they aid in mapping GNAI1 distribution in brain regions linked to neurotransmission. Ensuring antibody validation is critical, as cross-reactivity with homologous Gα subunits (e.g., GNAI2. GNAI3) can lead to false interpretations. Overall, GNAI1 antibodies are indispensable for unraveling the protein's functional and clinical significance.