THOC3抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Jurkat and PC3 cell lysates, Primary antibody: P10397(THOC3 Antibody) at dilution 1/1600, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 5 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10397(THOC3 Antibody) at dilution 1/85. (Original magnification: ×200)    

  
  

The THOC3 antibody is a crucial tool for studying the THO complex subunit 3 (THOC3), a component of the evolutionarily conserved THO complex involved in mRNA processing and export. The THO complex, comprising THOC1-THOC7 proteins, interacts with other mRNA export factors (e.g., ALYREF, UAP56) to form the Transcription-Export (TREX) complex, which couples transcription elongation with nuclear mRNA export. THOC3. a 33 kDa protein, plays a structural role in stabilizing the THO complex and ensuring proper mRNA splicing and transport. Dysregulation of THOC3 is linked to developmental defects, neurodevelopmental disorders, and cancers, as it influences the expression of genes critical for cell proliferation and differentiation.

THOC3 antibodies are widely used to investigate its expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and immunoprecipitation. These antibodies help elucidate THOC3's role in maintaining mRNA stability, preventing R-loop formation, and mediating cellular stress responses. Commercial THOC3 antibodies are typically raised against specific epitopes (e.g., recombinant human THOC3 fragments) and validated for specificity across human, mouse, and rat models. Research utilizing these antibodies has highlighted THOC3's importance in embryogenesis, neuronal function, and cancer progression, making it a biomarker of interest in both basic and clinical studies.