RFC5抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: 231 cell lysate, Primary antibody: P10381(RFC5 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    

  
  

**Background of RFC5 Antibody**

RFC5 (Replication Factor C Subunit 5) is a critical component of the RFC complex, a conserved AAA+ ATPase responsible for loading the proliferating cell nuclear antigen (PCNA) onto DNA during replication and repair. As part of the RFC1-5 heteropentamer, RFC5 plays a role in DNA damage recognition, replication fork progression, and cell cycle regulation. Its interaction with PCNA ensures processive DNA synthesis by DNA polymerases and facilitates error-free lesion bypass.

RFC5-specific antibodies are essential tools for studying DNA replication mechanisms, genome stability, and checkpoint signaling. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect RFC5 expression, localization, and protein interactions. Dysregulation of RFC5 has been linked to cancer, as altered replication fidelity contributes to genomic instability and tumorigenesis. Studies also suggest RFC5 overexpression in certain malignancies, highlighting its potential as a therapeutic target or biomarker.

Research employing RFC5 antibodies has clarified its role in cellular responses to replication stress, such as UV-induced damage or chemotherapy. Furthermore, RFC5 knockdown models reveal its necessity for viability, underscoring its fundamental role in DNA metabolism. Continued exploration of RFC5 may advance understanding of replication-associated diseases and inform anticancer strategies.