NFYA抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10373(NFYA Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

The NFYA antibody is a research tool designed to detect Nuclear Transcription Factor Y Subunit A (NF-YA), a critical component of the NF-Y heterotrimeric transcription factor complex. NF-Y, composed of NF-YA, NF-YB, and NF-YC subunits, binds to the CCAAT box, a conserved DNA motif in gene promoters, to regulate transcription. NF-YA contains an evolutionarily conserved DNA-binding domain and plays a central role in stabilizing the complex's interaction with DNA. It is ubiquitously expressed and implicated in diverse cellular processes, including cell cycle regulation, apoptosis, and stress responses. Dysregulation of NF-YA has been linked to cancers, metabolic disorders, and developmental defects.

NFYA antibodies are widely used in molecular biology to study NF-YA expression, localization, and function. They enable techniques like Western blotting, immunohistochemistry, chromatin immunoprecipitation (ChIP), and immunofluorescence. These antibodies are typically raised in rabbits or mice, with monoclonal and polyclonal variants available. Specificity validation via knockout controls or siRNA knockdown is essential due to potential cross-reactivity with homologous proteins. Researchers employ NFYA antibodies to explore its role in gene regulatory networks, disease mechanisms, and therapeutic targeting. Recent studies highlight NF-YA's involvement in cancer progression, stem cell differentiation, and metabolic reprogramming, underscoring its value as a biomarker or intervention target in precision medicine.