DNAJA4抗体—艾普蒂

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human breast cancer tissue lysate, Primary antibody: P04529(ACR Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

DNAJA4 antibody targets the DNAJA4 protein, a member of the Hsp40/DnaJ family of molecular chaperones. These proteins function as co-chaperones for Hsp70 ATPases, aiding in protein folding, disaggregation, and quality control by regulating Hsp70’s ATPase activity. DNAJA4. specifically, is characterized by its conserved J-domain that mediates interaction with Hsp70. Unlike other family members, DNAJA4 exhibits tissue-specific expression, with higher levels reported in the testis, brain, and certain cancers, suggesting roles in cell proliferation, stress response, and disease pathology.

Antibodies against DNAJA4 are widely used in research to study its expression patterns, subcellular localization (primarily cytoplasmic), and involvement in diseases such as cancer, neurodegenerative disorders, and conditions linked to protein misfolding. They enable techniques like Western blotting, immunohistochemistry, and immunofluorescence to explore DNAJA4’s regulatory mechanisms under stress conditions like heat shock or oxidative stress. Commercially available DNAJA4 antibodies are typically developed in hosts like rabbits or mice, using immunogens derived from recombinant protein fragments or specific epitopes. Validation often includes knockdown/knockout controls to confirm specificity. Research highlights DNAJA4’s potential as a biomarker or therapeutic target, particularly in malignancies where its dysregulation correlates with tumor progression or drug resistance.