LRAT抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: SP20 cell lysate, Primary antibody: P10317(LRAT Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P10317(LRAT Antibody) at dilution 1/55. (Original magnification: ×200)    

  
  

**Background of LRAT Antibodies**

LRAT (lecithin retinol acyltransferase) is a key enzyme in the visual cycle and vitamin A metabolism, catalyzing the esterification of all-*trans*-retinol into all-*trans*-retinyl esters within retinal pigment epithelial (RPE) cells. This reaction is critical for storing retinoids and maintaining retinal homeostasis. Dysregulation of LRAT activity is linked to retinal disorders like Stargardt disease, Leber congenital amaurosis, and age-related macular degeneration (AMD).

LRAT antibodies are immunological tools designed to detect and quantify LRAT protein expression in research and diagnostic settings. These antibodies are typically generated using peptide antigens derived from conserved regions of the human or murine LRAT protein. They enable the study of LRAT's role in retinoid metabolism, cellular localization in RPE cells, and its interaction with other visual cycle components.

In research, LRAT antibodies are utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess LRAT expression levels in disease models or genetic studies. For instance, they help evaluate LRAT deficiency in knockout mice or analyze LRAT upregulation in therapeutic interventions targeting retinal degeneration. Commercially available LRAT antibodies are often validated for specificity and sensitivity, though variability in performance across species or tissue types requires careful optimization.

Beyond basic research, LRAT antibodies hold potential in developing biomarker assays for retinal diseases and monitoring gene therapy outcomes aimed at restoring LRAT function in inherited blindness. Their application bridges mechanistic insights into vitamin A metabolism and translational efforts to treat LRAT-associated pathologies.