CSTF3抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: K562 and Jurkat cell lysates, Primary antibody: P10222(CSTF3 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 40 seconds    

  
  

The CSTF3 antibody targets the Cleavage Stimulation Factor subunit 3 (CSTF3), a critical component of the cleavage stimulation factor (CSTF) complex involved in mRNA 3'-end processing. CSTF3. also known as CstF-64. plays a central role in polyadenylation, a key step in mRNA maturation that influences transcript stability, nuclear export, and translation. It recognizes polyadenylation signals in nascent RNA and recruits other processing factors to ensure proper cleavage and poly-A tail addition. Dysregulation of CSTF3 has been linked to diseases, including cancers and neurological disorders, due to its role in alternative polyadenylation (APA), which can alter gene expression patterns.

CSTF3 antibodies are widely used in research to study RNA processing mechanisms, protein-RNA interactions, and disease-associated APA events. These antibodies enable techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect CSTF3 expression, localization, and interactions. Commercially available CSTF3 antibodies are typically validated for specificity using knockdown/knockout controls. However, batch variability and cross-reactivity with paralogs (e.g., CstF-64τ) require careful experimental optimization. Recent studies also explore CSTF3's potential as a therapeutic target, emphasizing the antibody's utility in both basic and translational research contexts.