RAB3IL1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: TM4 cell lysate, Primary antibody: P10135(RAB3IL1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 10 seconds    

  
  

The RAB3IL1 antibody is a tool used to detect Rab3A-interacting molecule-like protein 1 (RAB3IL1), a guanine nucleotide exchange factor (GEF) involved in regulating synaptic vesicle trafficking and neurotransmitter release. RAB3IL1. also known as guanine nucleotide exchange factor for Rab3A (GRAB), plays a critical role in synaptic transmission by activating Rab3A, a small GTPase essential for vesicle docking and exocytosis. This protein is predominantly expressed in neuronal tissues but is also found in secretory cells, suggesting broader roles in membrane trafficking pathways.

Antibodies targeting RAB3IL1 are utilized in research to study its localization, expression levels, and interactions within cellular pathways. They are commonly applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to elucidate its function in neurodevelopment, synaptic plasticity, and diseases such as neurodegenerative disorders or cancer. For instance, altered RAB3IL1 expression has been linked to glioblastoma and intellectual disability, highlighting its potential as a biomarker or therapeutic target.

Commercial RAB3IL1 antibodies are typically raised in hosts like rabbits or mice, with validation via knockout controls or siRNA silencing to confirm specificity. Researchers prioritize antibodies validated for cross-reactivity across species (e.g., human, mouse, rat) to support translational studies. Understanding RAB3IL1's role through such antibodies contributes to unraveling mechanisms of vesicle dynamics and cellular secretion, bridging gaps in neurobiology and disease pathology.