POLR3F抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: PC-3 and HepG2 cell lysates, Primary antibody: P10090(POLR3F Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 25 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10090(POLR3F Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P10090(POLR3F Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The POLR3F antibody targets the POLR3F protein, a subunit of RNA polymerase III (Pol III), a DNA-dependent enzyme responsible for transcribing small non-coding RNAs, including tRNAs, 5S rRNA, and other regulatory RNAs. POLR3F, also known as RPC6. is part of the Pol III core complex, contributing to its structural integrity and catalytic activity. This subunit plays a critical role in transcription initiation and elongation by stabilizing interactions with DNA and other polymerase components.

Antibodies against POLR3F are widely used in research to study Pol III assembly, function, and regulation. They enable detection of POLR3F expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding investigations into Pol III-related cellular processes, such as ribosome biogenesis and cell proliferation. Dysregulation of Pol III activity, including POLR3F dysfunction, has been linked to diseases like inherited neurodevelopmental disorders (e.g., hypomyelinating leukodystrophy) and cancers, where elevated Pol III activity is associated with tumor growth.

POLR3F antibodies also help explore tissue-specific expression patterns and post-translational modifications influencing Pol III activity. Researchers often validate antibody specificity using knockout cell lines or recombinant protein controls. As Pol III gains attention in disease mechanisms and therapeutic targeting, POLR3F antibodies remain essential tools for elucidating its biological and pathological roles.