ATP5PD抗体—艾普蒂

+ +

•  

   

     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-6: Mouse skeletal muscle tissue, Mouse kidney tissue, PC-3, Jurkat, HepG2 and Hela cell lysates, Primary antibody: P10089(ATP5PD Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P10089(ATP5PD Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P10089(ATP5PD Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The ATP5PD antibody targets the ATP synthase peripheral stalk subunit d, a critical component of mitochondrial ATP synthase (Complex V). This enzyme complex, located in the inner mitochondrial membrane, catalyzes ATP synthesis during oxidative phosphorylation. The peripheral stalk, including subunit d (encoded by the ATP5PD gene), stabilizes the complex and facilitates proton translocation-driven rotational mechanics essential for converting ADP to ATP.

ATP5PD antibodies are widely used in research to study mitochondrial function, energy metabolism, and diseases linked to ATP synthase dysfunction, such as neurodegenerative disorders, metabolic syndromes, and cancer. These antibodies enable detection and quantification of ATP5PD expression via techniques like Western blotting, immunofluorescence, and immunohistochemistry.

Commercial ATP5PD antibodies are typically raised in rabbits or mice using recombinant protein fragments or synthetic peptides. Validation includes specificity checks using knockdown/knockout models or tissue lysates with known expression levels. Researchers must optimize experimental conditions (e.g., dilution ratios, blocking buffers) due to potential cross-reactivity with homologous proteins or isoform variations.

Recent studies highlight ATP5PD's role in mitochondrial dynamics, apoptosis, and cellular stress responses, expanding its relevance beyond basic energy production. Reliable ATP5PD antibodies thus serve as vital tools for unraveling mitochondrial pathophysiology and therapeutic targets.