TMEM9抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P10030(TMEM9 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The TMEM9 antibody is a research tool used to study the transmembrane protein 9 (TMEM9), a conserved lysosomal and endosomal membrane-associated protein implicated in vesicle trafficking, lysosomal acidification, and V-ATPase assembly. TMEM9 is characterized by a single transmembrane domain and interacts with vacuolar ATPase (V-ATPase), a key regulator of intracellular pH. Its role in modulating V-ATPase activity links TMEM9 to cellular processes like autophagy, nutrient sensing, and tumor progression. Dysregulation of TMEM9 has been observed in cancers, including colorectal, hepatocellular, and breast cancers, where it promotes proliferation, metastasis, and chemoresistance.

TMEM9 antibodies are primarily used in Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect TMEM9 expression and localization in tissues or cell lines. They help validate TMEM9's overexpression in cancer models and its association with poor prognosis. Additionally, these antibodies aid in elucidating TMEM9's molecular mechanisms, such as its interaction with V-ATPase subunits or involvement in Wnt/β-catenin and mTOR signaling pathways. Commercial TMEM9 antibodies are typically raised against specific epitopes, such as the N-terminal or C-terminal regions, and require validation for species reactivity (e.g., human, mouse) and application-specific compatibility. Research using TMEM9 antibodies continues to explore its potential as a therapeutic target or biomarker in oncology and lysosome-related disorders.