SMUG1抗体—艾普蒂

+ +

•  

   

     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P10025(SMUG1 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

SMUG1 (Single-strand-selective Monofunctional Uracil-DNA Glycosylase 1) is a DNA repair enzyme belonging to the uracil-DNA glycosylase family. It primarily excises uracil residues from DNA formed through spontaneous deamination of cytosine or misincorporation during replication, playing a critical role in base excision repair (BER) pathways. Unlike other glycosylases, SMUG1 exhibits a preference for single-stranded DNA and is implicated in both nuclear and mitochondrial genome maintenance. Its activity is linked to genomic stability, aging, and cancer development, with dysregulation observed in various malignancies and neurodegenerative disorders.

SMUG1 antibodies are immunological tools developed to detect, quantify, and study the spatial-temporal expression of SMUG1 proteins in cells and tissues. These antibodies are widely utilized in techniques such as Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry. High-quality SMUG1 antibodies are validated for specificity using knockout cell lines or siRNA knockdown controls to minimize cross-reactivity with related glycosylases like UNG or TDG. Researchers employ them to investigate SMUG1's role in DNA damage responses, its interaction with repair pathway components, and its potential as a biomarker in cancer prognosis or therapeutic targeting. Commercial SMUG1 antibodies are typically raised in rabbits or mice, with recombinant proteins or peptide fragments serving as immunogens. Their applications span basic research in DNA repair mechanisms, clinical studies exploring SMUG1 expression patterns in tumors, and pharmacological screens for BER pathway modulators.