RPS27A抗体—艾普蒂

+ +

•  

   

     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HL60 cell, Mouse kidney tissue lysates, Primary antibody: P10012(RPS27A Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P10012(RPS27A Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human prostate cancer tissue using P10012(RPS27A Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The RPS27A antibody is a crucial tool for studying the multifunctional protein RPS27A, also known as ubiquitin-40S ribosomal protein S27a. RPS27A serves dual roles: as a component of the 40S ribosomal subunit involved in protein synthesis and as a precursor for ubiquitin, a key regulator of protein degradation via the ubiquitin-proteasome system. This small protein is encoded by the RPS27A gene and is post-translationally processed to generate mature ubiquitin and a residual ribosomal protein.

RPS27A antibodies are widely used to investigate its expression, localization, and functional interplay in cellular processes such as ribosome biogenesis, DNA repair, cell cycle regulation, and stress responses. These antibodies are essential in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in various disease contexts, including cancer, neurodegenerative disorders, and immune dysregulation. For example, altered RPS27A expression has been linked to tumor progression, chemoresistance, and viral infection responses.

Researchers often validate antibody specificity using knockout cell lines or siRNA-mediated knockdown to ensure accurate detection. Both polyclonal and monoclonal RPS27A antibodies are available, typically raised in hosts like rabbits or mice. Understanding RPS27A's roles through such antibodies provides insights into ribosome-ubiquitin crosstalk and potential therapeutic targets for diseases tied to proteostasis imbalance or ribosomal dysfunction.