POLR1D抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P09984(POLR1D Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09984(POLR1D Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

The POLR1D antibody is a research tool designed to detect and study the POLR1D protein, a subunit of RNA polymerase I and III complexes. RNA polymerase I (Pol I) and III (Pol III) are essential enzymes responsible for transcribing ribosomal RNA (rRNA) and small RNAs (e.g., tRNA, 5S rRNA), respectively, which are critical for ribosome biogenesis and cellular proliferation. POLR1D, encoded by the POLR1D gene, forms a heterodimer with POLR1C to stabilize the structural integrity of Pol I and Pol III. Dysregulation of POLR1D has been linked to developmental disorders and cancers, including Treacher Collins syndrome and autosomal dominant hearing loss, as well as tumorigenesis in various tissues.

POLR1D antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to evaluate POLR1D expression levels, subcellular localization, and interactions. These antibodies aid in understanding POLR1D’s role in transcription regulation, its involvement in diseases, and potential therapeutic targeting. Specificity and validation are crucial for POLR1D antibodies due to structural similarities among RNA polymerase subunits. Researchers often verify results using knockdown/knockout controls or orthogonal methods. Recent studies also explore POLR1D mutations in congenital disorders, underscoring the antibody’s utility in both basic research and clinical diagnostics.