COPE抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Hela, A375 and PC-3 cell lysates, Primary antibody: P09543(COPE Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09543(COPE Antibody) at dilution 1/25. (Original magnification: ×200)    

  
  

COPE antibodies target the ε-subunit of the COPI (coatomer protein complex I) complex, a key player in intracellular vesicular transport. The COPI complex, composed of seven subunits (α, β, β', γ, δ, ε, ζ), is primarily involved in retrograde trafficking within the Golgi apparatus and between the Golgi and the endoplasmic reticulum (ER). This trafficking ensures proper protein sorting, organelle maintenance, and cellular homeostasis. The ε-subunit (COPE) contributes to coatomer assembly and vesicle budding, playing a critical role in maintaining ER-Golgi architecture and regulating cargo selection.

COPE antibodies are widely used in cell biology research to study COPI-mediated transport mechanisms, protein localization, and organelle dynamics. They enable detection of COPE expression levels via techniques like Western blotting, immunofluorescence, and immunoprecipitation, aiding in investigations of secretory pathway dysregulation. Such studies are crucial for understanding diseases linked to COPI dysfunction, including certain cancers, neurodegenerative disorders (e.g., Alzheimer’s), and congenital glycosylation defects. Additionally, COPE antibodies help explore COPΙ's role in lipid homeostasis and autophagy. Their application in model organisms and cell lines has provided insights into evolutionary conservation of vesicular transport systems. By elucidating COPI-related mechanisms, these antibodies contribute to therapeutic strategies targeting membrane trafficking anomalies.