CDC7抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: 231, 293T, PC-3, Hela cell lysates, Primary antibody: P09465(CDC7 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds    

  
  

The cell division cycle 7 (CDC7) kinase is a serine/threonine kinase essential for initiating DNA replication during the S phase of the cell cycle. It functions by phosphorylating components of the replication machinery, such as the minichromosome maintenance (MCM) complex, to activate replication origins. CDC7 operates in a heterodimeric complex with its regulatory subunit, DBF4 (also called ASK), and its activity is tightly regulated throughout the cell cycle. Dysregulation of CDC7 has been linked to genomic instability and oncogenesis, making it a potential therapeutic target in cancers characterized by uncontrolled proliferation.

CDC7-specific antibodies are critical tools for studying its expression, localization, and activation status in both normal and pathological contexts. These antibodies enable detection of CDC7 protein levels via Western blotting, immunohistochemistry, or immunofluorescence, and can distinguish phosphorylated (active) forms using phospho-specific epitopes. Researchers also employ CDC7 antibodies to explore interactions with binding partners (e.g., DBF4) or assess the efficacy of CDC7 inhibitors in preclinical models. Given CDC7's role in DNA replication and its overexpression in various malignancies, such antibodies support efforts to develop targeted therapies, including small-molecule inhibitors currently under investigation for cancer treatment.