CALCOCO2抗体—艾普蒂

+ +

•  

   

     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal liver tissue lysate, Primary antibody: P09427(CALCOCO2 Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

The CALCOCO2 (Calcium-binding and coiled-coil domain-containing protein 2) antibody is a tool used to detect the CALCOCO2 protein, also known as NDP52 (Nuclear dot protein 52). This protein plays a critical role in selective autophagy, particularly in xenophagy (clearance of intracellular pathogens) and mitophagy (degradation of damaged mitochondria). CALCOCO2 acts as an autophagy receptor by recognizing ubiquitin-coated cargo, such as invading bacteria or dysfunctional organelles, and recruiting core autophagy machinery (e.g., LC3/GABARAP proteins) to initiate autophagosome formation. Its coiled-coil domains mediate protein-protein interactions, while its ubiquitin-binding domain targets tagged substrates.

CALCOCO2 antibodies are widely used in research to study autophagy pathways, host-pathogen interactions, and mitochondrial quality control. They enable detection of CALCOCO2 expression, localization, and interactions via techniques like Western blot, immunofluorescence, and co-immunoprecipitation. Dysregulation of CALCOCO2 has been implicated in neurodegenerative diseases, cancer, and infectious diseases, making it a focus for therapeutic exploration. Commercial CALCOCO2 antibodies are typically raised in hosts like rabbits or mice, with validation in knockout models to confirm specificity. These reagents are essential for elucidating the molecular mechanisms of autophagy and its role in cellular homeostasis.