USP14抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A172 and Jurkat cell lysates, Primary antibody: P09331(USP14 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

USP14 (Ubiquitin-specific protease 14) is a deubiquitinating enzyme (DUB) belonging to the ubiquitin-specific protease family. It plays a critical role in the ubiquitin-proteasome system (UPS), which regulates protein degradation and cellular homeostasis. USP14 is unique among DUBs as it associates with the 19S regulatory particle of the 26S proteasome, where it trims ubiquitin chains from substrates to either rescue proteins from degradation or facilitate their processing. This dual functionality positions USP14 as a key modulator of proteasomal activity and protein turnover.

Research has linked USP14 to various physiological and pathological processes. It is implicated in neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, where dysregulated protein aggregation occurs. USP14’s inhibition has been shown to enhance proteasome activity, potentially clearing toxic aggregates. Additionally, USP14 overexpression is observed in certain cancers, where it may promote cell survival by stabilizing oncoproteins, making it a therapeutic target. Small-molecule inhibitors like IU1 and its derivatives are being explored to modulate USP14 activity.

Antibodies targeting USP14 are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation to investigate USP14’s interaction partners, regulatory mechanisms, and disease associations. These antibodies help validate USP14’s role in cellular pathways and its potential as a biomarker or drug target in various pathologies.