UBE4A抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P09312(UBE4A Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P09312(UBE4A Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The UBE4A (Ubiquitination Factor E4A) antibody is a crucial tool for studying the UBE4A protein, a member of the UBE4 family of ubiquitin ligases involved in the ubiquitin-proteasome system (UPS). UBE4A plays a role in substrate recognition and degradation by catalyzing the formation of polyubiquitin chains, marking proteins for proteasomal breakdown. It is implicated in cellular processes like DNA repair, apoptosis, and neuronal development, with dysregulation linked to neurodegenerative disorders and cancers.

UBE4A antibodies are widely used in research applications such as Western blotting, immunoprecipitation, and immunohistochemistry (IHC) to detect protein expression, localization, and interaction partners. These antibodies are essential for exploring UBE4A's regulatory mechanisms, including its role in modulating pathways like the p53 signaling network or stress responses. Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies provide high specificity, aiding in distinguishing UBE4A from homologous family members (e.g., UBE4B).

Validated UBE4A antibodies are critical for ensuring experimental reproducibility. Commercial antibodies are typically raised in hosts like rabbits or mice, with validation using knockout cell lines or siRNA knockdown to confirm target specificity. Researchers prioritize antibodies with demonstrated cross-reactivity data across species (human, mouse, rat) and compatibility with specific experimental conditions. Reliable UBE4A antibodies advance understanding of its pathophysiological roles and potential therapeutic targeting in diseases.