UBE2D1抗体—艾普蒂

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Jurkat, 231 and LOVO cell lysates, Primary antibody: P09309(UBE2D1 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P09309(UBE2D1 Antibody) at dilution 1/240. (Original magnification: ×200)    

  
  

The UBE2D1 antibody is a crucial tool for studying the ubiquitin-proteasome system, a primary pathway for targeted protein degradation in eukaryotic cells. UBE2D1 (Ubiquitin-Conjugating Enzyme E2 D1) belongs to the E2 enzyme family, which works with E3 ligases to transfer ubiquitin molecules onto substrate proteins, marking them for proteasomal destruction. This enzyme is involved in diverse cellular processes, including DNA repair, cell cycle regulation, and stress response. Dysregulation of UBE2D1 has been implicated in cancer, neurodegenerative diseases, and immune disorders, making it a research focus for therapeutic targeting.

UBE2D1 antibodies are typically developed in host species like rabbits or mice using immunogenic peptides derived from conserved regions of the human UBE2D1 protein. These antibodies enable detection of endogenous UBE2D1 via techniques such as Western blotting, immunofluorescence, and immunoprecipitation. Specificity is validated using knockout cell lines or siRNA-mediated knockdown controls. Researchers utilize UBE2D1 antibodies to investigate its substrate interactions, tissue-specific expression patterns, and post-translational modifications. Commercial antibodies often provide data on cross-reactivity with orthologs (e.g., mouse, rat) and application-specific validation. As ubiquitination research expands, high-quality UBE2D1 antibodies remain essential for elucidating its role in disease mechanisms and cellular homeostasis.