ZNF354A抗体—艾普蒂

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P09240(ZNF354A Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P09240(ZNF354A Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The ZNF354A antibody is a tool used to study the ZNF354A protein, a member of the zinc finger protein (ZFP) family characterized by conserved C2H2-type zinc finger domains. ZNF354A, also known as RED1 or KRAB-ZFP93. functions as a transcription factor, primarily binding to DNA through its zinc finger motifs to regulate gene expression. It contains a KRAB (Krüppel-associated box) domain at its N-terminus, which is known to recruit transcriptional co-repressors like histone-modifying enzymes (e.g., HDACs), enabling chromatin remodeling and gene silencing. ZNF354A is implicated in diverse biological processes, including cell differentiation, apoptosis, and epigenetic regulation, with potential roles in cancer and developmental disorders.

Researchers employ ZNF354A antibodies in techniques such as Western blotting, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and immunofluorescence (IF) to investigate its expression, localization, and DNA-binding interactions. These antibodies are typically raised in hosts like rabbits or mice, using immunogenic peptides derived from ZNF354A's unique regions. Specificity is validated via knockout controls or peptide blocking assays. Studies using ZNF354A antibodies have revealed its involvement in repressing oncogenic pathways and maintaining cellular homeostasis, though its precise mechanisms remain under exploration. Its dysregulation has been observed in certain cancers, highlighting its therapeutic or diagnostic potential. Reliable commercial sources often provide these antibodies with detailed validation data to ensure reproducibility in experimental models.