ZKSCAN1抗体—艾普蒂

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human cerebrum tissue lysate, Primary antibody: P09223(ZKSCAN1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P09223(ZKSCAN1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The ZKSCAN1 antibody is a research tool designed to detect and study the ZKSCAN1 protein, a member of the SCAN domain-containing zinc finger transcription factor family. ZKSCAN1. also known as ZNF139. is implicated in transcriptional regulation, particularly in processes like autophagy, cell differentiation, and proliferation. It contains a conserved SCAN domain, which mediates protein-protein interactions, and multiple C2H2-type zinc finger motifs that facilitate DNA binding. Studies suggest ZKSCAN1 acts as a transcriptional repressor, potentially suppressing genes involved in autophagosome formation, thereby negatively regulating autophagy under nutrient-rich conditions.

ZKSCAN1 antibodies are typically produced in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are widely used in techniques such as Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to assess protein expression, subcellular localization, and DNA-binding activity. Research has linked ZKSCAN1 to cancer progression, with overexpression observed in certain malignancies, possibly promoting tumor growth by inhibiting apoptosis or autophagy-dependent cell death. Its role in neurological disorders and aging is also under exploration. Antibody validation often includes knockout controls to confirm specificity, ensuring reliable detection of the ~75 kDa protein. These tools remain critical for elucidating ZKSCAN1's biological functions and therapeutic potential.